1. Collect every drop of urine during each 24-hour period. It does not matter how much or little urine is passed each time, as long as every drop is collected.
2. Begin the urine collection in the morning after you wake up, after you have emptied your bladder for the first time.
3. Urinate (empty the bladder) for the first time and flush it down the toilet. Note the exact time (e.g. 8 am). This is noted as the ‘start time’ of the test.
4. Collect every drop of urine during the day and night in the collection bottle. (The paper container may of help to collect the urine for female patients before pouring into the collection bottle) Store the bottle at room temperature or in the refrigerator.
5. If you need to have a bowel movement, any urine passed with the bowel movement should be collected. Try not to include feces with the urine collection. If feces does get mixed in, do not try to remove the feces from the urine collection bottle. Also, do not put toilet paper in the collection container.
6. Finish by collecting the first urine passed the next morning, adding it to the collection bottle. This should be within 10 minutes before or after the time of the first morning void on the first day (which was flushed). In this example, you would try to void between 7:50 am and 8:10 am on the second day.
7. Discard the paper container and return the urine collection bottle to the clinic during working hours (Mondays to Sundays 9 am – 5 pm) or to the Laboratory directly during after working hours.
Reference: Up to date: Collection of a 24-hour Urine Specimen